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Part:BBa_I716015:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-07-10)


RFP without start ATG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 552
    Illegal AgeI site found at 664
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

I716015 (pBca9145-RFP_noATG)

ay013/14 on I716004       709  bp  (BglII, XhoI)
into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
prod I716015
---
ay013  cgacgAGATCTgcttcctccgaagacgttatc
ay014  gcataCTCGAGttaGGATCCttattaagcaccggtggag


Source

places

References